Journal: Frontiers in Immunology

Article Title: Delineating the Association Between Soluble CD26 and Autoantibodies Against G-Protein Coupled Receptors, Immunological and Cardiovascular Parameters Identifies Distinct Patterns in Post-Infectious vs. Non-Infection-Triggered Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

doi: 10.3389/fimmu.2021.644548

Figure Lengend Snippet: Parameters significantly correlating with sCD26 in male patients (onset stratified).

Article Snippet: Alpha1/2 adrenergic receptor (Alpha1-AR/alpha2-AR)-AAB, beta1-AR-/beta2-AR/beta3-AR-AAB, M3-mAChR-/M4-mAChR-AAB as well as Angiotensin-II-receptor type 1 (AT1-R)-AAB and Endothelin receptor A and B (ETA-R/ETB-R)-AAB were determined using ELISA technology by CellTrend GmbH (Luckenwalde, Germany).

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Delineating the Association Between Soluble CD26 and Autoantibodies Against G-Protein Coupled Receptors, Immunological and Cardiovascular Parameters Identifies Distinct Patterns in Post-Infectious vs. Non-Infection-Triggered Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

doi: 10.3389/fimmu.2021.644548

Figure Lengend Snippet: Parameters significantly correlating with sCD26 in the whole ME/CFS cohort (onset stratified).

Article Snippet: Alpha1/2 adrenergic receptor (Alpha1-AR/alpha2-AR)-AAB, beta1-AR-/beta2-AR/beta3-AR-AAB, M3-mAChR-/M4-mAChR-AAB as well as Angiotensin-II-receptor type 1 (AT1-R)-AAB and Endothelin receptor A and B (ETA-R/ETB-R)-AAB were determined using ELISA technology by CellTrend GmbH (Luckenwalde, Germany).

Techniques: Infection

Journal:

Article Title: Generation and Analysis of Siah2 Mutant Mice

doi: 10.1128/MCB.23.24.9150-9161.2003

Figure Lengend Snippet: Normal TRAF-, Vav1-, and OBF-1-mediated responses in Siah2 mutant mice and primary cells. (A) B-cell proliferation. Splenic white blood cells from 8-week-old wild-type or Siah2−/− mice were activated in vitro for 72 h with LPS (0.5, 1, 2, and 20 μg/ml) or anti-IgM (5 and 20 μg/ml) or by culture on irradiated 3T3 fibroblasts expressing CD40L (1,000 and 2,000 irradiated cells per well). Proliferation was analyzed by [3H]thymidine incorporation for the final 16 h of culture. Data represent the means + standard deviations (SD) (error bars) of triplicate activations from each of three mice of each genotype. (B) T-cell proliferation was analyzed as in panel A after stimulation of splenic white blood cells in 96-well plates with ConA (0.5 μg/ml) or by culture in wells coated with anti-CD3 (5 and 10 μg/ml) or anti-CD3 (5 μg/ml) plus anti-CD28 (10 μg/ml) antibodies. (C and D) Wild-type (•) or Siah2−/− (○) mice received intraperitoneal injections with 10 μg of the T-independent antigen NP-LPS (C) or with 100 μg of the T-dependent antigen NP-KLH precipitated on alum (D). Serum samples were obtained at time points after immunization and analyzed using an anti-NP and IgM-specific ELISA (C) or an anti-NP and IgG1-specific ELISA. Each circle in panel C represents the absorbance reading obtained from a 1:1,585 dilution of serum from an immunized mouse (the absorbance reading was linear with respect to dilution over the range 1:500 to 1:5,000), and each circle in panel D represents the absorbance reading obtained from a 1:10,000 dilution of serum from an immunized mouse (the absorbance reading was linear with respect to dilution over the range 1:3,170 to 1:31,700). Mean values are shown by horizontal lines. (E) Confluent BMM pooled from two wild-type or Siah2−/− mice were starved of M-CSF for 24 h prior to stimulation for a further 24 h with recombinant CSF-1 (10,000 U/ml) alone or in combination with LPS (0.1 μg/ml), poly(I) · poly(C) (pI.pC, 0.1 or 50 μg/ml), TNF-α (1 or 100 ng/ml), or IFN-β (100 U/ml). DNA synthesis was measured by incorporation of [3H]thymidine for the final 8 h of culture. Data represent means + SD (error bars) of triplicate stimulations.

Article Snippet: Cells were starved of M-CSF for 24 h by growth in the absence of L-cell-conditioned medium prior to restimulation with recombinant human CSF-1 (Chiron) with or without LPS ( E. coli 0111:B4; Sigma), poly(I) · poly(C) (Calbiochem), recombinant mouse TNF-α (BD Pharmingen), or beta interferon (IFN-β) (BD Pharmingen).

Techniques: Mutagenesis, In Vitro, Irradiation, Expressing, Enzyme-linked Immunosorbent Assay, Recombinant, DNA Synthesis